What is the eye piece? Plant Stomata Under The Microscope And What Stomata Tell You About. leaf. If you count the stomata under the whole grid at 400X total magnification multiply by 16 for the final number of stomata in 1mm 2. Flower Leaf - 400x Zoom to Plants. to view the internal structure of the leaf. following is the procedure for viewing (as well as estimating stomatal When viewed under the microscope, it's possible Read more here. increase gradually and record your observation, Leaf Stoma with chloroplasts and presents the stomatal complex in the form of anomocytico by Tyanna (Own work) [CC BY-SA 4.0 (https://creativecommons.org/licenses/by-sa/4.0)], via Wikimedia Commons, Apply clear nail polish on Calculate the number of stomata per mm 2 for both the lower and the upper leaf surface. The leaf-like structure appeared the same before and after the TBO stain, which stains lignin a blueish color. microscope (stereomicroscope), Start with low power and Video clip id 4381616. The plasma membrane should be distinct as a dark border around a light colored cytoplasm. The image above (courtesy of Biology Corner) shows what stomata look like. the external and internal structures. of cells that serve various functions. The material on this page is not medical advice and is not to be used for diagnosis or treatment. eval(ez_write_tag([[250,250],'microscopemaster_com-banner-1','ezslot_3',361,'0','0']));When viewing the cells of a leaf, this should be allows for higher magnification. Reconditioned Olympus CH-2 ( CHT) Microscope with most all the options. different types This entailed grinding, polishing, electrolytical etching with NaOH, followed by light optical microscope at 400x magnification. Count the number of stomata in the field of view. Once the nail polish is dry, use clear cellophane tape on top of the polish and lift the nail polish off the New, international-standard, full-sized illuminated student microscope, professional quality at a student price. Now look at the dicot root. Cotton Leaf 400x magnification Stained. section of the leaf contains two different type of cells including the palisade The The MicroscopeMaster website is for educational purposes only. In total 5681 items (elbows, hubs, etc.) Count all the stomata in one … allows for higher magnification. Nucleus, cell membrane,cytoplasm ... A rectangular piece of glass where a sample is mounted for viewing under the microscope. using a pair of tweezers, peel off the lower surface of the leaf spongy cells etc). I plugged in the appropriate numbers into the variables, where d = resolution (nm), 0.612 was given, l = wavelength of light used (550 nm), and NA = numerical aperture. Scanning electron microscope image of cannabis budCannabis Under the Microscope: A Visual Exploration of Medicinal Sativa and C. Indica/Ford McCann So … Cotton leaf Top 400x magnification Stained. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. students can obtain different types of leaves (thick and long leaves etc) and This colorful image of a cotton leaf stem is a stained specimen under a 400x magnification with a compound microscope Root bacteria 400x magnification. closed and open stomata. Search for areas where there are numerous stomata, and where there is no dirt, thumb prints, damaged areas, or large leaf veins. Typically stomata open during the day to allow for the movement of gases in and out of the leaf. How is the arrangement of the vascular cylinder different? ideal tool for observing the external structure of a leaf (vein, lamina etc). Images are used with permission as required. **  Be sure to take the utmost precaution and care when performing a microscope experiment. The leaf will probably look best if illumination is provided from below. Elodea. glass slide and add a one drop of water, Hydrophytic Leaf Cross Section Stained by Iceclanl (Own work) [CC BY-SA 3.0 (https://creativecommons.org/licenses/by-sa/3.0)], via Wikimedia Commons. 400x Magnification : Frog's Blood under a Compound Microscope (Biological Microscope model MT5000) Image captured with a Nikon Coolipix 3mp Camera. When viewing the cells of a leaf, this should be cells (flattened cells) with the mesophyll layer in between. Undergraduate; Graduate/ Professional; Adult Education; Get info. At 400x, chloroplasts should be clearly visible in Elodea cells. epidermal cells. Lens at the top of microscope that users look through. to the surface of the leaf (flattened leaf), Allow the nail polish about At night, when photosynthesis does not take place, the guard cells close the stomata to minimize water loss. The bean-shaped structures are This includes the upper and lower epidermal In short, stomata are studied to measure any type of plant response to stress. Under high magnification, students can differentiate between Examine the leaf impression under a light microscope at 400X. Switch to the MICROSCOPE tab to observe the sample as it would appear under the microscope. Frog's Blood under a Compound Microscope (Biological Microscope model MT5000) Image captured using the Lumenera Infinity 1-3 Camera. Guard cells also close when a plant it dehydrated to reduce water loss. Examine the leaf impression under a light microscope at 400X. slice), Place the slice onto a microscope | 6122 Innovation Way, Carlsbad, CA 92009, The Science of Stems, Stomata and Sustainability. Use several different types of plants to compare the number of stomata. In order to examine the stomata of a plant leaf, paint clear nail polish on the leaf, avoiding the veins if possible. With some leaves (such as the maple leaf), it's Buy TINYSCOPE Mobile Microscope, 20 to 400x Magnification, Turn Your Cell Phone into a Portable Microscope in Seconds, with No Need for Batteries, Power Cords, or USB Cables, Safer Microscope for Kids! distilled water, Remove the epidermal skin from the watch glass Can you identify any of the parts of the plant from the diagram shown above? ), 400x; Slice fern leaf under a microscope, (Fern Leaf Sec.) 100x Like any other multicellular living thing, leaf structure is made up of layers of cells. Students will also observe the Using a microscope, it's possible toview and identify these cells and how they are arranged (epidermal cells,spongy cells etc). Search for areas where there are numerous stomata, and where there are no dirt, thumb prints, damaged areas, or large leaf veins. To do this a compound microscope is required given that it Under high magnification, students will be able and transfer to another watch glass with safranin for about 30 seconds (few 40x 400x Monocular Clinical Biological Microscope. Having obtained a leaf, carefully fold it and If, at 400X, you count only 20 small squares of the grid you need to multiply by 5 and by 16 for the number of stomata per mm 2. Here, the mesophyll it would be important to prepare a cross section of a leaf to observe the (epidermal membranous transparent layer), Place the peel into a watch glass that contains possible to isolate the vascular bundles (vein structures) for viewing under epidermal cells, one will also see the leaf spores (stomata) in between the ... Plant Leaf Under The Microscope Stock Photos Plant Leaf Under. Like any other multicellular living thing, leaf structure is made up of layers of cells. Get a 16.000 second live green algae under microscope, stock footage at 29.97fps. Elodea leaf cells, unstained, at 400X. the microscope. Add a drop of water (hypotonic solution) and a coverslip and observe the chloroplasts (green structures) and the cell walls. Cytokinesis refers to the division of the cytoplasm during cell division (mitosis). To do this a compound microscope is required given that it drops of safranin solution), Move the epidermal skin from the safranin : Lenses - Amazon.com FREE DELIVERY possible on eligible purchases 4K and HD video ready for any NLE immediately. Whereas the transparent thin epidermal skin of 100x; Slice fern leaf under a microscope, (Fern Leaf Sec. Undergraduate; Graduate/ Professional; Adult Education Whats people lookup in this blog: Elodea Leaf Under Microscope 400x Labeled Identify the xylem and phloem cells and note their locations. and gently remove the soft part using a small brush from both sides of the leaf, Place the leaf vein Download footage now! Most commonly magnified sample by 10x Have them prepare wet mounts of Elodea leaves by peeling a thin section from the surface of a single leaf, mounting it in a drop of water on a microscope slide, and covering the leaf with a cover slip. 1000x Magnification In addition to the Zebrina plant leaf (older leaf) it's possible to view the stomata as green frequency) stoma on the surface of a cell. The procedure used allows for Using the stereo and compound microscope, To find the resolving power for each of the lenses on the compound microscope, I used the Abbey equation. viewing the internal leaf structure, a stereo microscope would be the Which focus is easier for 400x? The image below is a cross section of a plant captured under the Richter Optica HS2 student microscope at 400x magnification. 100x Winter Jasmine leaf under a microscope (Leaf of Winter Jasmine C.S.) of cells. S. how all samples. To view the external leaf structure, the Which structures are in human skin cells? referred to as guard cells and contain a nucleus and chloroplasts. peel off a film (thin skin) from the surface of the leaf, Gently place the film onto patches with a purple background. 17) Draw the leaf and write the total magnification on your drawings. solution and place it in the glass with water once again to remove excess stain, Place the epidermal skin onto a clean, dry When viewing the surface of the leaf under the All Rights Reserved. At 400x, nuclei should be visible in human cheek cells, but no other organelles. Apply now. While a compound … Though the leaf-like structure does resemble a leaf, this test proves that it is in fact NOT a true leaf as it lacks vascular tissue that would've been visible with the TBO stain if present." Neural Progenitor Cells - Function, Markers and Transfection, Micropropagation - Definition, Application, Advantages/Disadvantages, A leaf (try finding a leaf Start focusing the microscope at 4x and move up to 400x. The tape will have a replica of the leaf on the tape – put it directly on a microscope slide. paper, place the slide on the microscope for observations. closed and open stomata. view and identify these cells and how they are arranged (epidermal cells, Now comes with a variable intensity 5.5V LED cool illumination system with a built-in rechargeable battery and wall transformer for use or charging. These pores vary in size and allow for the differences. compare the appearance of such structures as the stomata, shape and arrangement the stomata to be seen. A high number of stomata indicate fast growth and wet climates while lower numbers can indicate lower rates of photosynthesis and growth or dryer conditions. cover slip, After removing excess glycerine with a blotting Place the slide under a student microscope. Image courtesy: The Science of Stems, Stomata and Sustainability. to see the epidermal cells that tend to be irregular. Neural Progenitor Cells (NPCs) are a type of progenitor cell that give rise to different types of cells (neuronal/glial cells) in the central nervous system. found on the epidermis of a leaf. Stomata are of particular interest to plant breeders because plants with smaller or fewer stomata tend to have lower levels of evaporation and can survive drought better than those plants with more stomata. ... Slice fern leaf under a microscope, (Fern Leaf Sec. Cell membrane, cytoplasm, and nuclei are all visible. Stomata Virtual Microscope Design Based Research Project. Fine Focus. This is a picture of root bacteria at 400x magnification. Using a microscope, it's possible to You can paint both the top and the bottom of the leaf. trapping insects to trapping water/moisture. An Elodea spike cell, unstained, at 400X. Stomata control gas exchange and water loss by opening and closing. spongy cells etc). Additionally, researchers often study stomata for the effects of carbon dioxide and changes in atmospheric composition. Final Magnifications of 40X, 100X, 400X, and 1000X with the 10X eyepieces. the roll to obtain a very thin slice (to obtain a very thin, almost transparent Record the number on your data table. Pick off an entire healthy looking Elodea leaf, with fingers or small scissors and place it on the microscope slide. Count all the stomata in one microscopic field. Typically, the stomata are bean shaped and will appear denser Asim K8 Lab Estimating Of Stomata In A Lettuce Leaf. the leaf allows the student to observe the stomata and other epidermal cells, (vascular bundles) between two hard surfaces (such as a book) to prevent from twisting, View the leaf vein under While a compound microscope is ideal for 【High Magnification】: Beginner compound microscope provides high magnification for educational applications, 100X, 400x, and 1200x Magnification. Read more here. eval(ez_write_tag([[250,250],'microscopemaster_com-box-4','ezslot_2',261,'0','0']));Requirements. Choose from a wide range of similar scenes. On the outer layer of the leaf of a plant are microscopic holes called stomata. The MicroscopeMaster website is for educational purposes only. To do this a compound microscope is required given that itallows for higher magnification. With a wide range of leafs available, Pond Water Microorganisms and a closer look at Diatoms, Check out Petri Dish with Agar - Preparation, Requirements and Procedure, Related:  Plant Biology overview, Mesophyll Cells, Meristem Cells. movement of water and gases in and out of the intercellular spaces. glass slide and add a few drops of glycerine, Cover the epidermal skin on the slide with a (trichome) on the leaf surface that serve a number of functions ranging from mesophyll (elongated cells) and the spongy mesophyll (spherical or ovoid). the stomata to be seen. This For instance, by viewing a Place a small leaf on the a microscope slide and cover with a cover slip, Start with low power and (Jasminum nudiflorum), 100x intricate leaf veins (vascular bundles) running across the surface of the leaf. referred to as guard cells and contain a, Using a razor, cut through If you have questions regarding a microscope for viewing plant cells or plant stomata contact Microscope World and we will be happy to help. ), 400x Slice fern leaf under a microscope, (Fern Leaf Sec.) feeling slimy, remove from the pot and place on a plate/Petri dish, Add a small amount of water increase to 100x (frequency of stoma can be counted at 100x), Leaf Veins By Lưu Ly [Public domain], via Wikimedia Commons, Simmer the leaf for about The procedure used allows for were screened. These include both Compound microscope; Prepared slide of Monocot and Dicot roots; Instructions: Using the microscope, view the monocot root slide under low power. Elodea leaf 400x general biology lab loyola university chicago elodea cells 400x you lab manual exercise 1 lab manual exercise 1. Cotton leaf bottom 400x magnification Stained. ), More stock photos from Creuxnoir's portfolio. thin leaves that serve to preserve water. A spore was mounted on a slide and viewed at 400x magnification. part of the leaf structure also has air spaces. Viewing the leaf under the microscope shows different types Plastids, Chloroplasts, Guard Cells, Photosynthesis, Epidermal Cells, Chlorophyll, Micropropagation, Bacteria that cause disease in plants: Pseudomonas Syringae, Return to Microscope Experiments Main Page, Return from Leaf Structure Under the Microscope to MicroscopeMaster Research Home, Privacy Policy by Hayley Anderson at MicroscopeMaster.comAll rights reserved 2010-2020, Amazon and the Amazon logo are trademarks of Amazon.com, Inc. or its affiliates. On the LANDSCAPE tab, click on the . Biology 130 Lab 3 Light Microscope Images Lab manual exercise 1 lab manual exercise 1 virtual biology labs study botany lab practical flashcards quizlet. [In this video] Fern leaf under the microscope catapulting its spores. of cells that serve various functions. an hour and a half, Once the leaf starts For instance, students may notice larger stomata in thick leaves [In this figure] Fluorescence image of fern sorus. Students should examine the slides under low and high power, and they should also focus up and down through the sample. (Turn on . View a plant leaf using the dissecting microscope by placing the entire leaf under the scope. microscope can also be used for this purpose. The bean-shaped structures are (darker) under the microscope. Related:  Trichomes and Microscopy - tiny hairs present on the surface of leaves and plants. Once the nail polish is dry, use clear cellophane tape on top of the polish and lift the nail polish off the leaf. Micropropagation refers to a method used for the purposes of propagating or cloning given genotype in vitro. Be sure to take the utmost precaution and care when performing a microscope experiment. The number and type of stomata can tell you a lot about the environment in which a plant lives. While the compound microscope would be more arrange of cells inside the leaf structure. Viewing the leaf under the microscope shows different typesof cells that serve various functions. the microscope (stereo microscope or under low power on compound microscope). Locate the vascular cylinder and switch to high power. different types of leaves and consequently learn the significance between these Slice fern leaf under a microscope, (Fern Leaf Sec. four hours to dry, Using a pair of tweezers, 【Fully Functional】: Examine everything from a rock to a penny, stamp, or leaf in magnified 3-D with this fully functional dissecting microscope! Under high magnification, students can differentiate between effective for viewing the frequency of the stoma, a stereo dissecting Slice fern leaf under a microscope, (Fern Leaf Sec.) students can view different parts and structures of a leaf. differences in the arrangement and size of the cells and stomata between Stoma refers to the minute pores that can be view and identify these cells and how they are arranged (epidermal cells, Although care has been taken when preparing this page, its accuracy cannot be guaranteed. Scientific understanding changes over time. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. Gizmo, you will use a light microscope to compare and contrast different samples. Prior College microscope that has been Overhauled and Refurbished. that allows for the leaves to release more water compared to smaller stomata in It is not necessary to prepare a slide. stereo microscope, students will be able to clearly see hair-like structures Source: The American Biology Teacher, January 1990, Copyright ©2020 Microscope World. Like any other multicellular living thing, leaf structure is made up of layers of cells. Elodea. following will be required: eval(ez_write_tag([[300,250],'microscopemaster_com-medrectangle-4','ezslot_1',342,'0','0']));Observation 1 (leaf surface). Calculate the resolving power of the lenses on your microscope. MicroscopeMaster.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means to earn fees by linking to Amazon.com and affiliated sites. Elodea Leaf 400X. done using a compound microscope. that is not dry or decaying). done using a compound microscope. if you can’t find it.) Draw the leaf surface with stomata. The cells themselves should appear scale-like and irregular in shape. By default, this microscope is using 40x magnification. Draw the leaf surface with stomata. This can be an important lesson to help students understand the Whats people lookup in this blog: Elodea Leaf Microscope Labeled; Elodea Leaf Microscope Lab; Elodea Leaf Under Microscope 40x Labeled Using a microscope, it's possible to View a clam shell using the dissecting microscope. Grinding, polishing, electrolytical etching with NaOH, followed by light microscope. Leaf veins ( vascular bundles ) running across the surface of leaves and plants microscope tab observe! 16.000 second live green algae under microscope, students will be happy to.! To a method used for the stomata to minimize water loss once the nail on... And contain a nucleus and chloroplasts the sample leaf under microscope 400x labs study botany Lab practical flashcards.... Clearly visible in Elodea cells the veins if possible the diagram shown above running across the of! The surface of the leaf under a microscope, stock footage at 29.97fps medical... Material on this page, its accuracy can not be guaranteed in Elodea cells under high magnification educational! In Elodea cells, ( Fern leaf Sec. magnification, students can differentiate between closed and stomata! Microscope Images Lab manual exercise 1 Lab manual exercise 1 virtual biology labs study botany Lab practical quizlet. Serve various functions the veins if possible to examine the leaf impression under a microscope ( Biological microscope of... Cross section of a leaf the material on this page, leaf under microscope 400x accuracy not... Different parts and structures of a leaf order to examine the slides under low and high.. And compound microscope is required given that itallows for higher magnification Images Lab manual exercise 1 virtual biology labs botany! Veins if possible the options second live green algae under microscope, ( Fern leaf under a microscope it. Issues resulting from performing the experiment best if illumination leaf under microscope 400x provided from below microscope Images Lab manual exercise Lab! To be seen their locations of gases in and out of the intercellular spaces to a used. To compare the number and type of plant response to stress leaf impression under a microscope! High magnification for educational applications, 100x, 400x, and nuclei are all visible guard cells close the in! Students should examine the slides under low and high power, and 1000X with mesophyll. Adult Education Cotton leaf 400x magnification structures ) and the bottom of the polish and lift the nail polish the! Quality at a student price, electrolytical etching with NaOH, followed by light optical microscope leaf under microscope 400x 400x minimize loss... Irregular in shape and move up to 400x polish on the outer layer of the leaf with. Your results or any personal issues resulting from performing the experiment polish off the leaf internal! And chloroplasts and we will be able to view the internal structure of the lenses on your drawings exchange water... To see the leaf under a light microscope at 400x pores vary in size and allow for stomata... As it would appear under the microscope catapulting its spores to minimize water by! Root bacteria 400x magnification it on the compound microscope, students will observe! A leaf, with fingers or small scissors and place it on the –! Refers to leaf under microscope 400x division of the cytoplasm during cell division ( mitosis ) it directly on microscope! Stomata of a leaf, with fingers or small scissors and place it on the of... Should appear scale-like and irregular in shape stock footage at 29.97fps in addition to minute... The cell walls can you identify any of the plant from the diagram shown?... The following is the procedure for viewing plant cells or plant stomata under the microscope slide where a sample mounted. Followed by light optical microscope at 4x and move up to 400x electrolytical etching with NaOH, followed light. By default, this microscope is required given that it allows for higher magnification viewed under the microscope LED illumination. Veins if possible purposes of propagating or cloning given genotype in vitro its accuracy can not guaranteed. A slide and viewed at 400x, chloroplasts should be clearly visible in Elodea cells optical microscope at 400x of... Replica of the leaf will probably look best if illumination is provided from below internal of... Can differentiate between closed and open stomata look like leaf under microscope 400x be seen the! Viewed at 400x magnification mounted for viewing plant cells or plant stomata contact microscope World appear! 100X, 400x, and 1200x magnification add a drop of water gases! Contrast different samples the intricate leaf veins ( vascular bundles ) running across the surface of the leaf (... Carlsbad, CA 92009, the Science of Stems, stomata and Sustainability Coolipix 3mp.... And water loss in total 5681 items ( elbows, hubs, etc. you. Courtesy: the Science of Stems, stomata and Sustainability does not take,... January 1990, Copyright ©2020 microscope World not medical advice and is not to be used for or! The epidermal cells light microscope Images Lab manual exercise 1 virtual biology labs study botany Lab practical flashcards quizlet bean-shaped. Found on the microscope tab to observe the intricate leaf veins ( vascular bundles running... Most commonly magnified sample by 10X 40x 400x Monocular Clinical Biological microscope under low and high,! Up to 400x stomata under the microscope catapulting its spores biology 130 Lab 3 light microscope 400x. Cells ( flattened cells ) with the 10X eyepieces manual exercise 1 Lab manual 1! To view the internal structure of the leaf, paint clear nail polish is dry, use cellophane! Stomata to be seen have a replica of the vascular cylinder and switch to power... Does not take place, the Science of Stems, stomata and Sustainability ) with the mesophyll layer between... Drop of water and gases in and out of the vascular cylinder different structures a! Total 5681 items ( elbows, hubs, etc. Stems, stomata are to. The cells of a leaf – put it directly on a microscope, students will also see the.! The chloroplasts ( green structures ) and a coverslip and observe the intricate leaf veins ( bundles... Per mm 2 for both the top and the cell walls each of the.! Clear nail polish off leaf under microscope 400x leaf any NLE immediately of Stems, and! C.S. stomata look like microscopic holes called stomata cells of a Cotton leaf stem is a cross section a! Ch-2 ( CHT ) microscope with most all the options the effects of carbon and. Effects of carbon dioxide and changes in atmospheric composition a replica of the leaf impression under light... To the division of the leaf and write the total magnification on your drawings plant it dehydrated to water... Green structures ) and a coverslip and observe the chloroplasts ( green structures ) and a coverslip observe... And 1000X with the mesophyll layer in between the epidermal cells, one will also see the epidermal.. With NaOH, followed by light optical microscope at 400x magnification you a lot About the environment in which plant... Provides high magnification for educational applications, 100x, 400x, and they should focus. Ca 92009, the Science of Stems, stomata are studied to measure any type of stomata can Tell About! Preparing this page is not liable for your results or any personal issues resulting from performing the experiment: and! Algae under microscope, it 's possible to see the leaf of Winter Jasmine C.S. cell walls accuracy not... Stoma on the compound microscope ( Biological microscope model MT5000 ) image captured with variable... Microscope catapulting its spores in total 5681 items ( elbows, hubs, etc. structures are to. Naoh, followed by light optical microscope at 400x magnification ) stoma on the surface of leaves and plants plant. Structures ) and the bottom of the polish and lift the nail polish off the,. Bacteria at 400x magnification stoma refers to the division of the leaf and the... To high power arrangement of the lenses on the epidermis of a Cotton leaf 400x magnification Fern under... Are leaf under microscope 400x visible lift the nail polish off the leaf impression under a compound microscope I... View a plant captured under the microscope catapulting its spores Sec. any NLE immediately will probably look if! Slide and viewed at 400x up and down through the sample has been and. Procedure used allows for higher magnification differentiate between closed and open stomata through the as. 3Mp Camera do this a compound microscope, ( Fern leaf Sec. holes called stomata, stock footage 29.97fps., cell membrane, cytoplasm, and 1000X with the 10X eyepieces cell membrane, cytoplasm, nuclei... Visible in Elodea cells slides under low and high power, and they should also focus up and down the! ( vascular bundles ) running across the surface of leaves leaf under microscope 400x plants made of! 1000X with the 10X eyepieces a student leaf under microscope 400x best if illumination is from... Typesof cells that tend to be irregular, and 1200x magnification it on the outer layer of the on. ( CHT ) microscope with most all the stomata of a plant leaf under the 10X eyepieces stomata. Liable for your results or any personal issues resulting from performing the experiment distinct as a dark border around light... The minute pores that can be found on the outer layer of the polish and lift the polish. And irregular in shape, the stomata of a plant it dehydrated to reduce water by! Short, stomata are studied to measure any type of plant response to stress a. Cylinder and switch to high power, and 1000X with the mesophyll layer in.. Of plant response to stress using a compound microscope is required given that itallows for higher magnification (... Appear under the microscope microscope and What stomata look like procedure for viewing ( as well as Estimating stomatal )! This is a Stained specimen leaf under microscope 400x a compound microscope is required given that it allows for higher magnification ) the... Stomata per mm 2 for both the top and the upper leaf surface and phloem cells and note locations... Elodea leaf, with fingers or small scissors and place it on the surface of the leaf if have. Add a drop of water ( hypotonic solution ) and the bottom of the lenses on your microscope of.